Clinical and Microbiological Outcomes Associated With Use of Platelet-Rich Plasma in Chronic Venous Leg Uclers: A Randomized Controlled Trial

Bruna Maiara Ferreira Barreto Pires, PhD, RN, Universidade Federal Fluminense, Niterói, Brazil. Beatriz Guitton Renaud Baptista de Oliveira, PhD, Universidade Federal Fluminense, Niterói, Brazil. Luciana Castilho Bokehi, GD, Universidade Federal Fluminense, Niterói, Brazil. Ronir Raggio Luiz, PhD, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Bernadete Teixeira Ferreira Carvalho, PhD, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Rosimere Ferreira Santana, PhD, Universidade Federal Fluminense, Niterói, Brazil. Priscilla Alfradique de Souza, PhD, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. Geraldo Renato de Paula, PhD, Universidade Federal Fluminense, Niterói, Brazil. Lenise Arneiro Teixeira, PhD, Universidade Federal Fluminense, Niterói, Brazil.

Journal of wound, ostomy, and continence nursing : official publication of The Wound, Ostomy and Continence Nurses Society. 2021;48(4):292-299
Abstract
PURPOSE To evaluate the susceptibility profiles of Staphylococcus aureus and Pseudomonas aeruginosa strains identified in chronic venous ulcers treated with platelet-rich plasma (PRP) and petrolatum gauze or petrolatum gauze alone and to quantitatively evaluate the bacterial load and biofilm-forming capacities of the detected S. aureus and P. aeruginosa strains. DESIGN Randomized controlled trial. SUBJECTS AND SETTING The convenience sample included 36 participants; 18 were allocated to the PRP combined with the petrolatum gauze group, and 18 were allocated to the control group, which was treated with petrolatum gauze alone. METHODS Thirty-six patients presenting with chronic venous ulcers were consecutively randomized to the PRP group (n = 18) or the petrolatum gauze control group (n = 18). We followed participants for 3 months during treatment and collected swab cultures from their wounds during weeks 1, 6, and 12 or until the wounds healed. The samples were analyzed using mass spectrometry. Antimicrobial susceptibility tests were performed using disk diffusion. RESULTS P. aeruginosa was identified in 39 (39%) of 100 samples, and S. aureus was detected in only 10 (10%) samples collected over the study period. At the end of the 12-week treatment period, the wound infections reduced in both the PRP (P = .0078) and control groups (P = .01). The microorganisms were susceptible to most of the tested antimicrobials. The PRP did not increase the bacterial load in the wounds. All S. aureus strains identified showed biofilm-forming capacities and were classified as weak biofilm producers. All P. aeruginosa strains produced biofilm, with 17 strains being classified as weak, 14 as moderate, and 8 as strong biofilm producers. CONCLUSIONS The PRP plus petrolatum gauze did not increase bacteriological growth or the microbial load in chronic venous ulcers compared with petrolatum gauze alone and could be a considered as an advanced treatment option for these types of chronic wounds.
Study details
Language : eng
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