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Effects of apheresis platelets treated with vitamin B2 photochemical technology on the release of white blood cells- and platelet-Derived Cytokines during Storage
Liu Y, Gai X, Zhang WJ, Shen H, Qiao WB, Zhang Y, Zhang CX, Ye h, Li HL, Zhuang YL
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017;25((4)):1212-1217.
Abstract
OBJECTIVE To investigate whether vitamin B2 photochemical pathogen reduction technology(PRT) treatment may lead to increase white cell- and platelet- derived cytokines release from platelets during storage. METHODS Sixty milliliters of leukodepleted apheresis platelets were collected from 20 healthy donors, then were divided into 2 parts: one part (30 ml) remained untreated to serve as control, while the other part was treated with vitamin B2-UVB photo-chemical technology as experimental group. During 7 d of storage under standard blood bank conditions, platelet coun-ting (PC), platelet distribution width (PDW), mean platelet volume (MPV), white cell-derived cytokines (IL-1beta, IL-2, IL-6, IL-8, TNF-alpha and IFN-gamma) and platelet-derived cytokines (CCL3, CCL5, TGF-beta-1 and PF4), P-selectin and phosphatidyl serine (PS) were analyzed on day 1, 3, 5 and 7 of storage, respectively. RESULTS No signi-ficant differences were observed on PC, PDW and MPV between the experimental and control groups, respectively. The higher levels of platelet-derived cytokines were detected and reached a plateau after 5-7 days of storage, and the cyto-kines showed significant increase in experimental group compared with the control group. PS expression increased signi-ficantly in experimental group as compared with control group on day 3, 5 and 7 of storage, respectively. The accumula-tion of P-selectin was significant higher in experimental group than that in control group on day 5 and 7 of storage (P<0.05). The white cell-derived cytokines were not elevated by PRT treatment during 7 days of storage. CONCLUSION The PRT-treated platelets are the main source of released cytokines during storage of PRT treatment. The levels of platelet-derived cytokines reach a plateau after 5-7 days of storage, most likely due to accelerated platelet activation and apoptosis.