1.
Immune modulation and microchimerism after unmodified versus leukoreduced allogeneic red blood cell transfusion in cancer patients: results of a randomized study
Lapierre V, Aupérin A, Robinet E, Ferrand C, Oubouzar N, Tramalloni D, Saas P, Debaene B, Lasser P, Tiberghien P
Transfusion. 2007;47((9):):1691-9.
Abstract
BACKGROUND Transfusion of red blood cells (RBCs) has been associated with immunomodulatory effects. Persistence of donor cells in the recipient may be contributive. STUDY DESIGN AND METHODS A randomized single-center trial was conducted to compare microchimerism and immune responses in 35 patients undergoing cancer surgery and transfused perioperatively with either unmodified RBCs (UN-RBCs, n = 18) or leukoreduced RBCs (LR-RBCs, n = 17). Biologic parameters included microchimerism assessment peripheral blood mononuclear cell (PBMNC) phenotyping, cytokine production by stimulated PBMNCs, FoxP3 gene expression, and T-cell repertoire (TCR) analysis. RESULTS Microchimerism was documented in 8 of 18 patients after UN-RBC transfusion while absent after LR-RBC transfusion (0/17; p = 0. 001). After UN-RBC transfusion, microchimerism was associated with increased interleukin (IL)-10 production (p = 0. 02), reduced TCR alteration (p = 0. 04), and reduced CD56+ cell counts (p = 0. 02) when compared to recipients without evidence for microchimerism. FoxP3 gene expression did not differ significantly between both treatment groups nor with the presence or absence of microchimerism in the UN-RBC group. Finally, after an initial early decrease after surgery and transfusion, IL-12 production increased and more significantly so after UN-RBC transfusion versus LR-RBC transfusion (p = 0. 05). CONCLUSION UN-RBC-induced microchimerism is associated with specific immunomodulatory effects in cancer patients who received transfusions during surgery.
2.
Immune competence and microchimerism after unmodified versus leuko-reduced allogeneic red blood cells transfusion in cancer patient: results of a randomized study
Lapierre V, Auperin A, Ferrand C, Oubouzar N, Robinet E, Tramalloni D, Saas P, Tiberghien P
Blood. 2002;100((11, Pt 2):): Abstract No. 1093.
3.
Prevalence of hepatitis C after bone marrow transplantation for solid tumours in children included in a randomised study from 1985 to 1987 comparing prophylactic versus therapeutic transfusion of platelet concentrates
Oubouzar N, Hartmann O, Dussaix E, Benhamou E, Tramalloni D, Valteau-Couanet D, Lapierre V
Bone Marrow Transplantation. 2001;27((Suppl S1):):S207.. Abstract No. P559.