1.
Effects of apheresis platelets treated with vitamin B2 photochemical technology on the release of white blood cells- and platelet-Derived Cytokines during Storage
Liu Y, Gai X, Zhang WJ, Shen H, Qiao WB, Zhang Y, Zhang CX, Ye h, Li HL, Zhuang YL
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017;25((4)):1212-1217.
Abstract
OBJECTIVE To investigate whether vitamin B2 photochemical pathogen reduction technology(PRT) treatment may lead to increase white cell- and platelet- derived cytokines release from platelets during storage. METHODS Sixty milliliters of leukodepleted apheresis platelets were collected from 20 healthy donors, then were divided into 2 parts: one part (30 ml) remained untreated to serve as control, while the other part was treated with vitamin B2-UVB photo-chemical technology as experimental group. During 7 d of storage under standard blood bank conditions, platelet coun-ting (PC), platelet distribution width (PDW), mean platelet volume (MPV), white cell-derived cytokines (IL-1beta, IL-2, IL-6, IL-8, TNF-alpha and IFN-gamma) and platelet-derived cytokines (CCL3, CCL5, TGF-beta-1 and PF4), P-selectin and phosphatidyl serine (PS) were analyzed on day 1, 3, 5 and 7 of storage, respectively. RESULTS No signi-ficant differences were observed on PC, PDW and MPV between the experimental and control groups, respectively. The higher levels of platelet-derived cytokines were detected and reached a plateau after 5-7 days of storage, and the cyto-kines showed significant increase in experimental group compared with the control group. PS expression increased signi-ficantly in experimental group as compared with control group on day 3, 5 and 7 of storage, respectively. The accumula-tion of P-selectin was significant higher in experimental group than that in control group on day 5 and 7 of storage (P<0.05). The white cell-derived cytokines were not elevated by PRT treatment during 7 days of storage. CONCLUSION The PRT-treated platelets are the main source of released cytokines during storage of PRT treatment. The levels of platelet-derived cytokines reach a plateau after 5-7 days of storage, most likely due to accelerated platelet activation and apoptosis.
2.
Evaluation of the advantages of platelet concentrates stored at 4 degrees C versus 22 degrees C
Yang J, Yin W, Zhang Y, Sun Y, Ma T, Gu S, Gao Y, Zhang X, Yuan J, Wang W
Transfusion. 2017;58((3):):736-747
Abstract
BACKGROUND Platelet (PLT) storage at cold temperatures (4 degrees C) can reduce bacterial contamination and lower the risk of transfusion-related complications. We compared the effects of 22 and 4 degrees C storage conditions for PLTs to further explore the efficiency of hemostasis in acute bleeding and extended PLT shelf life. STUDY DESIGN AND METHODS Manually prepared PLTs (PLT concentrates in plasma, not PLT additive solution) were stored at 4 and 22 degrees C. The PLT counts, scanning electronic microscope observations, blood gas indices, biochemical indices, PLT aggregative function, and surface CD62P expression were monitored and compared between the groups. RESULTS There was no obvious change in PLT counts between Day 21 at 4 degrees C and Day 5 at 22 degrees C. PLTs stored at 4 degrees C for 10 to 14 days were dramatically activated, had rough surfaces, and showed a significant degree of long pseudopodia formation. The pH of the PLTs on Day 5 was lower at 22 degrees C than at 4 degrees C, while the lactate dehydrogenase and lactic acid levels in the former group were significantly higher (p < 0.005). The maximum aggregation rates induced by collagen and arachidonic acid in the PLTs stored at 4 degrees C for 5 days remained higher than 80%, while the rates induced by four inducers in the PLTs stored at 22 degrees C were less than 5%. PLTs stored at 4 degrees C for 10 to 14 days showed higher surface expression of PAC-1 and CD62P. CONCLUSION PLT counts, cellular morphologies, PLT membranes, cytoplasmic structures, aggregation rates, and hemostatic PLT function stored at 4 degrees C for 10 to 14 days were better than those stored at 22 degrees C for 5 days.